[Evaluation of novel nucleic acid detection kit for Mycoplasma pneumoniae].
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi
; 28(2): 85-89, 2018 Dec 25.
Article
en Ja
| MEDLINE
| ID: mdl-30630334
ABSTRACT
For diagnosis of Mycoplasma pneumoniae infection, highly sensitive and rapid diagnosis is important. Because antibiotics are limited for the treatment of M. pneumoniae infection. In this study, we evaluated new rapid nucleic acid detection kit for M. pneumoniae. This kit does not require excessive pretreatment of specimens and molecular diagnosis of M. pneumoniae is possible within 40 min. Using 120 nasopharyngeal specimens, we compared this kit with a commercially available molecular diagnostic reagent (LAMP). 51 of 120 cases were M. pneumoniae positive, and the results of both assays were all consistent. In addition, sequencing of 23S rRNA gene was performed on 51 cases positive for M. pneumoniae. As a result, macrolide resistance mutation (2063A>G) was observed in 19 cases (37.3%). The gene mutations estimated by this kit coincided completely with the sequencing. In conclusion, new rapid nucleic acid detection kit could detect M. pneumoniae with the same sensitivity as other molecular diagnostics, in a simple process.
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Base de datos:
MEDLINE
Asunto principal:
Neumonía por Mycoplasma
/
ARN Ribosómico 23S
/
Mycoplasma pneumoniae
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Ja
Revista:
Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi
Asunto de la revista:
MICROBIOLOGIA
Año:
2018
Tipo del documento:
Article