Highly Conductive Nucleotide Analogue Facilitates Base-Calling in Quantum-Tunneling-Based DNA Sequencing.
ACS Nano
; 13(5): 5028-5035, 2019 05 28.
Article
en En
| MEDLINE
| ID: mdl-30888791
ABSTRACT
Quantum-tunneling-based DNA sequencing is a single molecular technology that has great potential for achieving facile and high-throughput DNA sequencing. In principle, the sequence of DNA could be read out by the time trace of the tunnel current that can be changed according to molecular conductance of nucleobases passing through nanosized gap electrodes. However, efficient base-calling of four genetic alphabets has been seriously impeded due to the similarity of molecular conductance among canonical nucleotides. In this article, we demonstrate that replacement of canonical 2'-deoxyadenosine (dA) with a highly conductive dA analogue, 7-deaza dA, could expand the difference of molecular conductance between four genetic alphabets. Additionally, systematic evaluation of molecular conductance using a series of dA and dG analogues revealed that molecular conductance of the nucleotide is highly dependent on the HOMO level. Thus, the present study demonstrating that signal characteristics of the nucleotide can be modulated based on the HOMO level provides a widely applicable chemical approach and insight for facilitation of single molecular sensing as well as DNA sequencing based on quantum tunneling.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia de ADN
/
Emparejamiento Base
/
Nucleótidos
Idioma:
En
Revista:
ACS Nano
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón