Residual Negative Pressure in Vacuum Blood-Collection Tube and Hemolysis in Pediatric Blood Specimens.
Lab Med
; 51(1): 41-46, 2020 Jan 02.
Article
en En
| MEDLINE
| ID: mdl-31185079
ABSTRACT
OBJECTIVE:
To determine a method to reduce specimen hemolysis rates in pediatric blood specimens.METHODS:
A total of 290 blood specimens from pediatric patients were classiï¬ed into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer. Also, we performed a paired test to measure the concentration of free hemoglobin in specimens from 25 randomly selected healthy adult volunteers, using a direct spectrophotometric technique.RESULTS:
The hemolytic rate of capped specimens was 2-fold higher than that of uncapped specimens. We found significant differences for LDH. Also, there was a significant difference in the concentration of free hemoglobin in the random-volunteers test.CONCLUSIONS:
Eliminating the residual negative pressure of vacuum blood-collection tubes was effective at reducing the macrohemolysis and/or microhemolysis rate.Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Recolección de Muestras de Sangre
/
Hemólisis
Límite:
Adolescent
/
Adult
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Child
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Female
/
Humans
/
Male
Idioma:
En
Revista:
Lab Med
Año:
2020
Tipo del documento:
Article
País de afiliación:
China