Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus.
Methods Mol Biol
; 2066: 217-234, 2020.
Article
en En
| MEDLINE
| ID: mdl-31512220
Induced pluripotent stem (iPS) cells are important tools for studying differentiation and for use in patient-specific disease modeling. We present a detailed method for the reprogramming of primary human fibroblasts to induced pluripotent stem cells using Sendai virus. These procedures allow for the efficient generation of multiple high-quality feeder-independent iPS cell lines for a given human fibroblast line. The iPS cell lines generated by this protocol can be used in a variety of differentiation and gene expression studies, as well as in genetic manipulations.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
/
Virus Sendai
/
Células Madre Pluripotentes Inducidas
/
Cultivo Primario de Células
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos