Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis.
Nat Commun
; 10(1): 4392, 2019 09 26.
Article
en En
| MEDLINE
| ID: mdl-31558725
ABSTRACT
The targeted endocytic recycling of the T cell receptor (TCR) to the immunological synapse is essential for T cell activation. Despite this, the mechanisms that underlie the sorting of internalised receptors into recycling endosomes remain poorly understood. To build a comprehensive picture of TCR recycling during T cell activation, we developed a suite of new imaging and quantification tools centred on photoactivation of fluorescent proteins. We show that the membrane-organising proteins, flotillin-1 and -2, are required for TCR to reach Rab5-positive endosomes immediately after endocytosis and for transfer from Rab5- to Rab11a-positive compartments. We further observe that after sorting into in Rab11a-positive vesicles, TCR recycles to the plasma membrane independent of flotillin expression. Our data suggest a mechanism whereby flotillins delineate a fast Rab5-Rab11a endocytic recycling axis and functionally contribute to regulate the spatial organisation of these endosomes.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Endosomas
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Receptores de Antígenos de Linfocitos T
/
Membrana Celular
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Proteínas de Unión al GTP rab
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Proteínas de Unión al GTP rab5
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Endocitosis
Límite:
Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Australia