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Biosynthesis of polyketides by two type III polyketide synthases from Aloe barbadensis.
Liu, Ai-Jing; Xie, Ke-Bo; Liu, Yu-Yu; Sui, Song-Yang; Chen, Ri-Dao; Chen, Da-Wei; Liu, Ji-Mei; Yang, Lin; Dai, Jun-Gui.
Afiliación
  • Liu AJ; College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China.
  • Xie KB; State Key Laboratory of Bioactive Substance and Function of Natural Medicines; CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, B
  • Liu YY; State Key Laboratory of Bioactive Substance and Function of Natural Medicines; CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, B
  • Sui SY; College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China.
  • Chen RD; State Key Laboratory of Bioactive Substance and Function of Natural Medicines; CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, B
  • Chen DW; State Key Laboratory of Bioactive Substance and Function of Natural Medicines; CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, B
  • Liu JM; State Key Laboratory of Bioactive Substance and Function of Natural Medicines; CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, B
  • Yang L; College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China.
  • Dai JG; State Key Laboratory of Bioactive Substance and Function of Natural Medicines; CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs; NHC Key Laboratory of Biosynthesis of Natural Products, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, B
J Asian Nat Prod Res ; 22(5): 434-443, 2020 May.
Article en En | MEDLINE | ID: mdl-31791147
ABSTRACT
Various bioactive polyketides have been found in Aloe barbadensis. However, the polyketide synthases (PKSs), which participate in biosynthesis of polyketides in A. barbadensis remain unknown. In this study, two type III PKSs (AbPKS1 and AbPKS2) were identified from A. barbadensis. AbPKS1 and AbPKS2 were able to utilize malonyl-CoA to yield heptaketides (TW93a and aloesone) and octaketides (SEK4 and SEK4b), respectively. AbPKS1 also exhibited catalytic promiscuity in recognizing CoA thioesters of aromatics to produce unusual polyketides. What Is more, a whole cell biocatalysis system with the capability of producing 26.4 mg/L of SEK4/SEK4b and 2.1 mg/L of aloesone was successfully established.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Aloe / Policétidos Idioma: En Revista: J Asian Nat Prod Res Asunto de la revista: BOTANICA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Aloe / Policétidos Idioma: En Revista: J Asian Nat Prod Res Asunto de la revista: BOTANICA / QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: China