Capsids of hepatitis B virus e antigen with authentic C termini are stabilized by electrostatic interactions.
FEBS Lett
; 594(6): 1052-1061, 2020 03.
Article
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| MEDLINE
| ID: mdl-31792961
ABSTRACT
The hepatitis B virus e antigen, an alternative transcript of the core gene, is a secreted protein that maintains viral persistence. The physiological form has extended C termini relative to Cp(-10)149, the construct used in many studies. To examine the role of the C termini, we expressed the constructs Cp(-10)151 and Cp(-10)154, which have additional arginine residues. Both constructs when treated with reductant formed capsids more efficiently than Cp(-10)149. These capsids were also substantially more stable, as measured by thermal denaturation and resistance to urea dissociation. Mutagenesis suggests that electrostatic interactions between the additional arginine residues and glutamate residues on adjacent subunits play a role in the extra stabilization. These findings have implications for the physiological role and biotechnological potential of this protein.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Virus de la Hepatitis B
/
Cápside
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Antígenos e de la Hepatitis B
Idioma:
En
Revista:
FEBS Lett
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos