Detection of CRISPR adaptation.
Biochem Soc Trans
; 48(1): 257-269, 2020 02 28.
Article
en En
| MEDLINE
| ID: mdl-32010936
ABSTRACT
Prokaryotic adaptive immunity is built when short DNA fragments called spacers are acquired into CRISPR (clustered regularly interspaced short palindromic repeats) arrays. CRISPR adaptation is a multistep process which comprises selection, generation, and incorporation of prespacers into arrays. Once adapted, spacers provide immunity through the recognition of complementary nucleic acid sequences, channeling them for destruction. To prevent deleterious autoimmunity, CRISPR adaptation must therefore be a highly regulated and infrequent process, at least in the absence of genetic invaders. Over the years, ingenious methods to study CRISPR adaptation have been developed. In this paper, we discuss and compare methods that detect CRISPR adaptation and its intermediates in vivo and propose suppressing PCR as a simple modification of a popular assay to monitor spacer acquisition with increased sensitivity.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Escherichia coli
/
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas
/
Sistemas CRISPR-Cas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biochem Soc Trans
Año:
2020
Tipo del documento:
Article
País de afiliación:
Rusia