Amyloid ß-induced Mesenteric Inflammation in an Alzheimer's Disease Transgenic Mouse Model.
Curr Alzheimer Res
; 17(1): 52-59, 2020.
Article
en En
| MEDLINE
| ID: mdl-32048974
ABSTRACT
BACKGROUND:
Alzheimer's disease (AD) is a neurodegenerative disorder histopathologically characterized by the accumulation of amyloid ß (Aß) peptides and inflammation associated with activated microglia. These features are well investigated in the central nervous system using AD-model mice; however, peripheral inflammation in these mice has not been investigated well.OBJECTIVE:
We evaluated the inflammatory responses, especially myeloid dendritic cells (mDCs), in peripheral lymphoid tissues in AD-model mice to determine their association with Aß deposition.METHODS:
We collected lymphocytes from mesenteric lymphoid nodes (MLNs) and Peyer's patches (PPs) of 5×FAD transgenic mice used as an AD model. Lymphocytes were analyzed using a flow cytometer to characterize mDCs and T cells. Collected lymphocytes were treated with Aß1-42 ex vivo to evaluate the inflammatory response.RESULTS:
We observed elevated levels of inflammatory cytokines and chemokines including interleukin (IL)-12 and macrophage inflammatory protein-1α in mDCs from MLNs and PPs and reduced levels of programmed death-ligand-1, an immunosuppressive co-stimulatory molecule, on the surface of mDCs from 5×FAD mice. Additionally, we found increases in interferon (IFN)-γ-producing CD4- or CD8- positive T cells in MLNs were increased in 5×FAD mice. Moreover, ex vivo treatment with Aß peptides increased the production of IL-12 and IFN-γ by lymphocytes from 5×FAD mice.CONCLUSION:
The present study showed that pro-inflammatory mDC and T cells were induced in MLNs and PPs of 5×FAD mice.Palabras clave
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1
Base de datos:
MEDLINE
Asunto principal:
Linfocitos
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Péptidos beta-Amiloides
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Citocinas
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Enfermedad de Alzheimer
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Inflamación
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Mesenterio
Límite:
Animals
Idioma:
En
Revista:
Curr Alzheimer Res
Asunto de la revista:
NEUROLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón