Salt gradient chromatographic separation of chondroitin sulfate disaccharides.
J Chromatogr A
; 1619: 460979, 2020 May 24.
Article
en En
| MEDLINE
| ID: mdl-32093904
ABSTRACT
In the present study, we describe the development of a fast, 2-step salt gradient for analysis of chondroitin sulfate disaccharides. Using salt gradients, which is somewhat unusual in HILIC-based separations, provides relatively fast chromatography with excellent sensitivity (15 min cycle time, 10-20 fmol/µL detection, 30-50 fmol/µL quantitation limit), and good linearity. The efficiency of the new method is demonstrated by measuring human tissue slices of healthy, cirrhotic, and cancerous liver samples. Preliminary results show major differences among the quantity and sulfation pattern of the various sample types.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Cloruro de Sodio
/
Sulfatos de Condroitina
/
Cromatografía Líquida de Alta Presión
/
Disacáridos
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
2020
Tipo del documento:
Article
País de afiliación:
Hungria