A low-cost smartphone controlled portable system with accurately confined on-chip 3D electrodes for flow-through cell electroporation.
Bioelectrochemistry
; 134: 107486, 2020 Aug.
Article
en En
| MEDLINE
| ID: mdl-32179452
ABSTRACT
Microscale flow-through electroporation at DC voltage has advantages in delivering small molecules. Yet, electroporation based on constant voltage are liable to generate electrolysis products which limits the voltage-operating window. Parallel on-chip 3D electrodes with close and uniform spacing are required to cut down voltage as well as provide enough electric field for electroporation. Here we present a simple electrode fabrication method based on capillary restriction valves in Z-axis to achieve parallel 3D electrodes with controllable electrode spacing in PDMS chips. With electrodes accurately placed in close range, a low voltage of only 1.5 V can generate enough electric field (>400 V/cm) to make cell membrane permeable. Squeeze flow is introduced to produce higher electric field (>800 V/cm) at a fixed voltage for more efficient electroporation. Benefit from the electrode fabrication method and application of squeeze flow, we develop a smartphone controlled microfluidic electroporation system which integrate functions of sample injection, pressure regulating, real-time observation and constant DC power supply. The system is used to electroporate two cell lines, showing a permeabilization percentage of 63% for HEK-293 cells and 58% for CHO-K1 cells with optimal parameters. Thus, the portable microfluidic system provides a cost-effective and user-friendly flow-through cell electroporation platform.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Electroporación
/
Costos y Análisis de Costo
/
Dispositivos Laboratorio en un Chip
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Teléfono Inteligente
Tipo de estudio:
Health_economic_evaluation
Límite:
Humans
Idioma:
En
Revista:
Bioelectrochemistry
Asunto de la revista:
BIOQUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China