Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail.
Methods Cell Biol
; 156: 259-270, 2020.
Article
en En
| MEDLINE
| ID: mdl-32222222
ABSTRACT
This book chapter describes the use of exogenous application of lysyl oxidase (LOX) and bone morphogenetic protein-1 (BMP1) to enhance collagen synthesis and deposition from fibroblasts in culture. The protocol includes the generation of human embryonic kidney (HEK) 293 cell lines overexpressing human LOX and BMP1 constructs in order to obtain supernatants enriched in these factors. Incubation of fibroblast monolayers with these conditioned media strongly increases the capacity of these cells to deposit collagen onto the insoluble extracellular matrix. We also describe the use of these decellularized fibroblast-derived matrices as a substrate for the growth and differentiation of mesenchymal stem cells.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Colágeno
/
Proteína Morfogenética Ósea 1
/
Proteína-Lisina 6-Oxidasa
Límite:
Humans
Idioma:
En
Revista:
Methods Cell Biol
Año:
2020
Tipo del documento:
Article
País de afiliación:
España