Brazilian red propolis extract enhances expression of antioxidant enzyme genes in vitro and in vivo.
Biosci Biotechnol Biochem
; 84(9): 1820-1830, 2020 Sep.
Article
en En
| MEDLINE
| ID: mdl-32490727
ABSTRACT
Brazilian red propolis reportedly has reactive oxygen species (ROS) scavenging effects in vitro, but the cellular mechanisms remain unclear. In the present study, the effects of an ethanol extract of Brazilian red propolis (EERP) on the Nrf2-ARE intracellular antioxidant pathway were examined in vitro and in vivo. EERP and its constituents transactivated the reporter gene through the ARE sequence and enhanced the expression of Nrf2-regulated genes in HEK293 cells. It also increased Nrf2 protein in the nucleus, which was partially inhibited by kinase inhibitors. Furthermore, EERP suppressed ROS generation and cytotoxicity induced by tert-butyl hydroperoxide. In vivo, orally administered EERP increased the expression of Nrf2-regulated genes in mice liver. These results suggest that EERP is a potential resource for preventing oxidative stress-related diseases as an Nrf2 inducer.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Própolis
/
Extractos Vegetales
/
Regulación Enzimológica de la Expresión Génica
/
Antioxidantes
Límite:
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón