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Isolation of Three Lycorine Type Alkaloids from Rhodolirium speciosum (Herb.) Ravenna Using pH-Zone-Refinement Centrifugal Partition Chromatography and Their Acetylcholinesterase Inhibitory Activities.
Correa, Diana Isabel; Pastene-Navarrete, Edgar; Bustamante, Luis; Baeza, Marcelo; Alarcón-Enos, Julio.
Afiliación
  • Correa DI; Laboratorio de Farmacognosia, Dpto. de Farmacia, Facultad de Farmacia, Unidad de Desarrollo Tecnológico, UDT. P.O. Box 237, Universidad de Concepción, PC4030000 Concepción, Chile.
  • Pastene-Navarrete E; Laboratorio de Farmacognosia, Dpto. de Farmacia, Facultad de Farmacia, Unidad de Desarrollo Tecnológico, UDT. P.O. Box 237, Universidad de Concepción, PC4030000 Concepción, Chile.
  • Bustamante L; Laboratorio de Síntesis y Biotransformación de Productos Naturales, Dpto. Ciencias Básicas, Universidad del Bio-Bio, PC3780000 Chillan, Chile.
  • Baeza M; Dpto. de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, PC4030000 Concepción, Chile.
  • Alarcón-Enos J; Dpto. Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, PC4030000 Concepción, Chile.
Metabolites ; 10(8)2020 Jul 28.
Article en En | MEDLINE | ID: mdl-32731456
Preparative separation of three lycorine type alkaloids from Rhodolirum speciosum (Amaryllidaceae) was successfully carried out using pH-zone-refinement centrifugal partition chromatography (CPC) using the solvent system methyl-tert-butyl ether/acetonitrile/water (4:1:5, v/v/v) in descending mode. Using this system, Alkaloid 1 (165.7 mg, 88.2%, purity), 2 (60.1 mg, 97.7% purity) and 3 (12.3 mg, 84.4% purity) were obtained in one step. For structure elucidation, the pure alkaloids were subjected to spectroscopy analysis using nuclear magnetic resonance experiments (1H-NMR, 13C-NMR) and gas chromatography coupled with mass spectrometry (GC-MS). Alkaloids 1, 2, and 3 were identified as 1-O-acetyl-5,6-dehydrolycorine, 1-O-acetyl-lycorine, and 1,2-O-diacetyl-5,6-dehydrolycorine, respectively. The acetylcholinesterase inhibitory activity of these alkaloids was IC50 151.1 µg/mL, IC50 203.5 µg/mL, IC50 470.0 µg/mL, and IC50 17.1 µg/mL, respectively.
Palabras clave

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Metabolites Año: 2020 Tipo del documento: Article País de afiliación: Chile

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Metabolites Año: 2020 Tipo del documento: Article País de afiliación: Chile