Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity.
Commun Biol
; 3(1): 429, 2020 08 06.
Article
en En
| MEDLINE
| ID: mdl-32764731
ABSTRACT
The Eph family of receptor tyrosine kinases is crucial for assembly and maintenance of healthy tissues. Dysfunction in Eph signaling is causally associated with cancer progression. In breast cancer cells, dysregulated Eph signaling has been linked to alterations in receptor clustering abilities. Here, we implemented a single-cell assay and a scoring scheme to systematically probe the spatial organization of activated EphA receptors in multiple carcinoma cells. We show that cancer cells retain EphA clustering phenotype over several generations, and the degree of clustering reported for migration potential both at population and single-cell levels. Finally, using patient-derived cancer lines, we probed the evolution of EphA signalling in cell populations that underwent metastatic transformation and acquisition of drug resistance. Taken together, our scalable approach provides a reliable scoring scheme for EphA clustering that is consistent over multiple carcinomas and can assay heterogeneity of cancer cell populations in a cost- and time-effective manner.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Carcinoma
/
Familia de Multigenes
/
Receptores de la Familia Eph
/
Análisis de la Célula Individual
Límite:
Humans
Idioma:
En
Revista:
Commun Biol
Año:
2020
Tipo del documento:
Article
País de afiliación:
Singapur