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Porous Silicon Nanoparticles Embedded in Poly(lactic-co-glycolic acid) Nanofiber Scaffolds Deliver Neurotrophic Payloads to Enhance Neuronal Growth.
Zuidema, Jonathan M; Dumont, Courtney M; Wang, Joanna; Batchelor, Wyndham M; Lu, Yi-Sheng; Kang, Jinyoung; Bertucci, Alessandro; Ziebarth, Noel M; Shea, Lonnie D; Sailor, Michael J.
Afiliación
  • Zuidema JM; Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
  • Dumont CM; Department of Biomedical Engineering, University of Miami, 1251 Memorial Drive, Coral Gables, FL 33146, USA.
  • Wang J; Materials Science and Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
  • Batchelor WM; Department of Biomedical Engineering, University of Miami, 1251 Memorial Drive.
  • Lu YS; Materials Science and Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
  • Kang J; Department of Nanoengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, CA.
  • Bertucci A; Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
  • Ziebarth NM; Department of Biomedical Engineering, University of Miami, 1251 Memorial Drive.
  • Shea LD; Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, MI 48109, USA.
  • Sailor MJ; Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Adv Funct Mater ; 30(25)2020 Jun 18.
Article en En | MEDLINE | ID: mdl-32982626
Scaffolds made from biocompatible polymers provide physical cues to direct the extension of neurites and to encourage repair of damaged nerves. The inclusion of neurotrophic payloads in these scaffolds can substantially enhance regrowth and repair processes. However, many promising neurotrophic candidates are excluded from this approach due to incompatibilities with the polymer or with the polymer processing conditions. This work provides one solution to this problem by incorporating porous silicon nanoparticles (pSiNPs) that are pre-loaded with the therapeutic into a polymer scaffold during fabrication. The nanoparticle-drug-polymer hybrids are prepared in the form of oriented poly(lactic-co-glycolic acid) nanofiber scaffolds. We test three different therapeutic payloads: bpV(HOpic), a small molecule inhibitor of phosphatase and tensin homolog (PTEN); an RNA aptamer specific to tropomyosin-related kinase receptor type B (TrkB); and the protein nerve growth factor (NGF). Each therapeutic is loaded using a loading chemistry that is optimized to slow the rate of release of these water-soluble payloads. The drug-loaded pSiNP-nanofiber hybrids release approximately half of their TrkB aptamer, bpV(HOpic), or NGF payload in 2, 10, and >40 days, respectively. The nanofiber hybrids increase neurite extension relative to drug-free control nanofibers in a dorsal root ganglion explant assay.
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Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Adv Funct Mater Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Base de datos: MEDLINE Idioma: En Revista: Adv Funct Mater Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos