WJMSCs intervention may relieve intrauterine adhesions in female rats via TGFβ1mediated Rho/ROCK signaling inhibition.
Mol Med Rep
; 23(1)2021 01.
Article
en En
| MEDLINE
| ID: mdl-33179074
ABSTRACT
Estrogen is a commonly used hormone in the adjuvant treatment of intrauterine adhesion (IUA), which can promote endometrial growth. Stem cell transplantation has also been reported to promote endometrial regeneration in IUA due to its potential differentiative capacity. Human Wharton's jelly mesenchymal stem cells (WJMSCs) are isolated from the umbilical cord, possess strong selfrenewal and proliferative abilities, and are hypoimmunogenic and nontumorigenic. Therefore, the present study aimed to investigate the therapeutic effects and underlying mechanism of WJMSCs transplantation with estrogen treatment, separately or as a combined therapy, on IUA. The IUA model was established using the ethanol damage method. A total of 50 SpragueDawley female rats were randomly divided into the control, IUA model, WJMSCs treatment, estrogen treatment and WJMSCs+ estrogen treatment groups (n=10/group). WJMSCs were injected three times at 5day intervals. IUA rats in the estrogen group received 0.2 mg/kg estrogen through intragastric administration, once every 2 days for 8 weeks. Morphological changes were evaluated by hematoxylineosin staining. Immunohistochemical evaluations of pankeratin, vimentin, transforming growth factor (TGF)ß1, RhoA, RhoB, RhoC, Rhoassociated coiledcoilcontaining protein kinase (ROCK)I, and ROCKII expression were performed in uterine tissue. After treatment, the uterine specimens were observed to have increased uterine thickness and gland numbers in all treatment groups compared with the IUA group; however, the degree of restoration in the independent WJMSCs and estrogen treatment groups was better than in the combined treatment group. Immunohistochemical analysis demonstrated that pankeratin expression was increased, and RhoA, ROCKI and TGFß1 expression was significantly inhibited in the WJMSCs and WJMSCs + estrogen treatment groups compared with the IUA group; however, the expression levels of these proteins were similar among all treatment groups. No change in vimentin expression was detected in any treatment group. The expression levels of RhoB, RhoC and ROCKII were clearly not affected by WJMSCs intervention alone. In conclusion, transplantation of WJMSCs may repair endometrial damage in IUA rats via TGFß1mediated inhibition of RhoA/ROCKI signaling.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Enfermedades Uterinas
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Adherencias Tisulares
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Estrógenos
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Factor de Crecimiento Transformador beta1
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Células Madre Mesenquimatosas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2021
Tipo del documento:
Article