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Gene expression and in vitro replication of bovine gammaherpesvirus type 4.
Romeo, F; Louge-Uriarte, E; Gonzalez-Altamiranda, E; Delgado, S; Pereyra, S; Morán, P; Odeón, A; Pérez, S; Verna, Andrea E.
Afiliación
  • Romeo F; Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Godoy Cruz 2370, C1425FQD, Buenos Aires, Argentina.
  • Louge-Uriarte E; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 7600, Mar del Plata, Argentina.
  • Gonzalez-Altamiranda E; Grupo de Sanidad Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), 7620, Balcarce, Argentina.
  • Delgado S; Grupo de Sanidad Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), 7620, Balcarce, Argentina.
  • Pereyra S; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina.
  • Morán P; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 7600, Mar del Plata, Argentina.
  • Odeón A; Grupo de Sanidad Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), 7620, Balcarce, Argentina.
  • Pérez S; Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Sede Tandil, Buenos Aires, Argentina.
  • Verna AE; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 7600, Mar del Plata, Argentina.
Arch Virol ; 166(2): 535-544, 2021 Feb.
Article en En | MEDLINE | ID: mdl-33403475
ABSTRACT
In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Replicación Viral / Expresión Génica / Herpesvirus Bovino 4 Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Arch Virol Año: 2021 Tipo del documento: Article País de afiliación: Argentina

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Replicación Viral / Expresión Génica / Herpesvirus Bovino 4 Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Arch Virol Año: 2021 Tipo del documento: Article País de afiliación: Argentina