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Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109.
Torres, Daniela; Mongiardini, Elías; Donadío, Florencia; Donoso, Raúl; Recabarren-Gajardo, Gonzalo; Gualpa, José; Spaepen, Stijn; Defez, Roberto; Lopez, Gastón; Bianco, Carmen; Cassán, Fabricio.
Afiliación
  • Torres D; Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: dtorres@exa.unrc.edu.ar.
  • Mongiardini E; Laboratorio de Interacciones Rizobios y Soja. Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas. Universidad Nacional de La Plata, La Plata, Argentina. Electronic address: mongiardini@biol.unlp.edu.ar.
  • Donadío F; Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: fdonadio@exa.unrc.edu.ar.
  • Donoso R; Programa Institucional de Fomento a la Investigación, Desarrollo e Innovación (PIDi), Universidad Tecnológica Metropolitana, Santiago, Chile; Center of Applied Ecology and Sustainability (CAPES), Santiago, Chile. Electronic address: radonoso@uai.cl.
  • Recabarren-Gajardo G; Bioactive Heterocycles Synthesis Laboratory, BHSL, Departamento de Farmacia, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Santiago, Chile; Centro Interdisciplinario de Neurociencias, Pontificia Universidad Católica de Chile, Santiago, Chile. Electronic address: grecab
  • Gualpa J; Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: jgualpa@exa.unrc.edu.ar.
  • Spaepen S; Katholieke Universiteit Leuven. Leuven, Belgium.
  • Defez R; Institute of Biosciences and Bioresources, National Research Council of Italy, UOS Naples, Italy. Electronic address: roberto.defez@ibbr.cnr.it.
  • Lopez G; Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: glopez@exa.unrc.edu.ar.
  • Bianco C; Institute of Biosciences and Bioresources, National Research Council of Italy, UOS Naples, Italy. Electronic address: carmen.bianco@ibbr.cnr.it.
  • Cassán F; Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: fcassan@exa.unrc.edu.ar.
Res Microbiol ; 172(3): 103814, 2021.
Article en En | MEDLINE | ID: mdl-33539931
ABSTRACT
Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and ß) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Bradyrhizobium / Redes y Vías Metabólicas / Ácidos Indolacéticos Idioma: En Revista: Res Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Bradyrhizobium / Redes y Vías Metabólicas / Ácidos Indolacéticos Idioma: En Revista: Res Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2021 Tipo del documento: Article