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A needle-type micro-sampling device for collecting nanoliter sap sample from plants.
Gao, Panpan; Kasama, Toshihiro; Godonoga, Maia; Ogawa, Atsushi; Sone, Chiharu; Komine, Masashi; Endo, Yoshishige; Koide, Tetsushi; Miyake, Ryo.
Afiliación
  • Gao P; Microfluidic Integrated Circuits Research Laboratory, Department of Bioengineering, School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan.
  • Kasama T; Microfluidic Integrated Circuits Research Laboratory, Department of Bioengineering, School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan.
  • Godonoga M; Microfluidic Integrated Circuits Research Laboratory, Department of Bioengineering, School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan.
  • Ogawa A; Department of Biological Production, Akita Prefectural University, Akita, 010-0195, Japan.
  • Sone C; Department of Biological Production, Akita Prefectural University, Akita, 010-0195, Japan.
  • Komine M; Department of Biological Production, Akita Prefectural University, Akita, 010-0195, Japan.
  • Endo Y; Microfluidic Integrated Circuits Research Laboratory, Department of Bioengineering, School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan.
  • Koide T; Research Institute for Nanodevice and Bio Systems, Hiroshima University, Hiroshima, 739-0046, Japan.
  • Miyake R; Microfluidic Integrated Circuits Research Laboratory, Department of Bioengineering, School of Engineering, The University of Tokyo, 113-8656, Tokyo, Japan. trmiyake@mail.ecc.u-tokyo.ac.jp.
Anal Bioanal Chem ; 413(11): 3081-3091, 2021 May.
Article en En | MEDLINE | ID: mdl-33733702
ABSTRACT
In plant research, measuring the physiological parameters of plants is vital for understanding the behavior and response of plants to changes in the external environment. Plant sap analysis provides an approach for elucidating the physiological condition of plants. However, to facilitate accurate sap analysis, a sampling device capable of collecting sap samples from plants is required. In this paper, a minimally invasive, needle-type micro-sampling device capable of collecting nanoliter (~ 91 nL) quantities of sap from plants is described. The developed micro-sampling system showed great reproducibility (3%) in experiments designed to assess sampling performance. As a proof of concept, sap samples were collected continuously from target plants with the micro-sampling system, and the dynamic changes in potassium ions, plant hormones and sugar levels inside plants were analyzed. The results demonstrated the feasibility of the micro-sampling device and its potential for developing a measurement system for plant research in the future.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Plantas / Manejo de Especímenes / Agujas Idioma: En Revista: Anal Bioanal Chem Año: 2021 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Plantas / Manejo de Especímenes / Agujas Idioma: En Revista: Anal Bioanal Chem Año: 2021 Tipo del documento: Article País de afiliación: Japón