A needle-type micro-sampling device for collecting nanoliter sap sample from plants.
Anal Bioanal Chem
; 413(11): 3081-3091, 2021 May.
Article
en En
| MEDLINE
| ID: mdl-33733702
ABSTRACT
In plant research, measuring the physiological parameters of plants is vital for understanding the behavior and response of plants to changes in the external environment. Plant sap analysis provides an approach for elucidating the physiological condition of plants. However, to facilitate accurate sap analysis, a sampling device capable of collecting sap samples from plants is required. In this paper, a minimally invasive, needle-type micro-sampling device capable of collecting nanoliter (~ 91 nL) quantities of sap from plants is described. The developed micro-sampling system showed great reproducibility (3%) in experiments designed to assess sampling performance. As a proof of concept, sap samples were collected continuously from target plants with the micro-sampling system, and the dynamic changes in potassium ions, plant hormones and sugar levels inside plants were analyzed. The results demonstrated the feasibility of the micro-sampling device and its potential for developing a measurement system for plant research in the future.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Plantas
/
Manejo de Especímenes
/
Agujas
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón