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Capturing human trophoblast development with naive pluripotent stem cells in vitro.
Io, Shingo; Kabata, Mio; Iemura, Yoshiki; Semi, Katsunori; Morone, Nobuhiro; Minagawa, Atsutaka; Wang, Bo; Okamoto, Ikuhiro; Nakamura, Tomonori; Kojima, Yoji; Iwatani, Chizuru; Tsuchiya, Hideaki; Kaswandy, Belinda; Kondoh, Eiji; Kaneko, Shin; Woltjen, Knut; Saitou, Mitinori; Yamamoto, Takuya; Mandai, Masaki; Takashima, Yasuhiro.
Afiliación
  • Io S; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan; Japan Society for the Promotion of Science, Tokyo 102-0083, Japan.
  • Kabata M; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Iemura Y; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Semi K; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Morone N; MRC Toxicology Unit, University of Cambridge, Cambridge CB2 1QR, UK.
  • Minagawa A; Department of Cell Growth and Differentiation, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Wang B; Department of Cell Growth and Differentiation, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Okamoto I; Department of Anatomy and Cell Biology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto 606-8501, Japan.
  • Nakamura T; Department of Anatomy and Cell Biology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto 606-8501, Japan; The HAKUBI Center for Advanced Research, Kyoto University, Kyoto 606-8501, Japan.
  • Kojima Y; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Department of Anatomy and Cell Biology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto 606-8501, Japan.
  • Iwatani C; Research Center for Animal Life Science, Shiga University of Medical Science, Shiga 520-2192, Japan.
  • Tsuchiya H; Research Center for Animal Life Science, Shiga University of Medical Science, Shiga 520-2192, Japan.
  • Kaswandy B; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Kondoh E; Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.
  • Kaneko S; Department of Cell Growth and Differentiation, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Woltjen K; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan.
  • Saitou M; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Department of Anatomy and Cell Biology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto 606-8501, Japan.
  • Yamamoto T; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan; Institute for the Advanced Study of Human Biology (ASHBi), Kyoto University, Kyoto 606-8501, Japan; AMED-CREST, AMED, Tokyo 100-0004, Japan; Medical Risk Avoidance Based on iPS Cells Team, RIKEN Center for Advanced
  • Mandai M; Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.
  • Takashima Y; Department of Life Science Frontiers, CiRA, Kyoto University, Kyoto 606-8507, Japan. Electronic address: y.takashima@cira.kyoto-u.ac.jp.
Cell Stem Cell ; 28(6): 1023-1039.e13, 2021 06 03.
Article en En | MEDLINE | ID: mdl-33831365
ABSTRACT
Trophoblasts are extraembryonic cells that are essential for maintaining pregnancy. Human trophoblasts arise from the morula as trophectoderm (TE), which, after implantation, differentiates into cytotrophoblasts (CTs), syncytiotrophoblasts (STs), and extravillous trophoblasts (EVTs), composing the placenta. Here we show that naïve, but not primed, human pluripotent stem cells (PSCs) recapitulate trophoblast development. Naive PSC-derived TE and CTs (nCTs) recreated human and monkey TE-to-CT transition. nCTs self-renewed as CT stem cells and had the characteristics of proliferating villous CTs and CTs in the cell column of the first trimester. Notably, although primed PSCs differentiated into trophoblast-like cells (BMP4, A83-01, and PD173074 [BAP]-treated primed PSCs [pBAPs]), pBAPs were distinct from nCTs and human placenta-derived CT stem cells, exhibiting properties consistent with the amnion. Our findings establish an authentic paradigm for human trophoblast development, demonstrating the invaluable properties of naive human PSCs. Our system provides a platform to study the molecular mechanisms underlying trophoblast development and related diseases.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Trofoblastos / Células Madre Pluripotentes Límite: Female / Humans / Pregnancy Idioma: En Revista: Cell Stem Cell Año: 2021 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Trofoblastos / Células Madre Pluripotentes Límite: Female / Humans / Pregnancy Idioma: En Revista: Cell Stem Cell Año: 2021 Tipo del documento: Article País de afiliación: Japón