Structural Investigations of Human A2M Identify a Hollow Native Conformation That Underlies Its Distinctive Protease-Trapping Mechanism.

Harwood, Seandean Lykke; Lyngsø, Jeppe; Zarantonello, Alessandra; Kjøge, Katarzyna; Nielsen, Peter Kresten; Andersen, Gregers Rom; Pedersen, Jan Skov; Enghild, Jan J

Harwood, Seandean Lykke; Lyngsø, Jeppe; Zarantonello, Alessandra; Kjøge, Katarzyna; Nielsen, Peter Kresten; Andersen, Gregers Rom; Pedersen, Jan Skov; Enghild, Jan J.

Afiliación

Harwood SL; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark; Global Research Technologies, Novo Nordisk A/S, Måløv, Denmark.
Lyngsø J; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark; Department of Chemistry, Aarhus University, Aarhus, Denmark.
Zarantonello A; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Kjøge K; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Nielsen PK; Global Research Technologies, Novo Nordisk A/S, Måløv, Denmark.
Andersen GR; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Pedersen JS; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark; Department of Chemistry, Aarhus University, Aarhus, Denmark. Electronic address: jsp@chem.au.dk.
Enghild JJ; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark; Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark. Electronic address: jje@mbg.au.dk.

Mol Cell Proteomics ; 20: 100090, 2021.

Article en En | MEDLINE | ID: mdl-33964423

Asunto(s)

Péptido Hidrolasas/química; alfa-Macroglobulinas/química; Células HEK293; Humanos; Espectrometría de Masas/métodos; Microscopía Electrónica/métodos; Mutación; Conformación Proteica; Proteínas Recombinantes/química; Dispersión del Ángulo Pequeño; alfa-Macroglobulinas/genética

Palabras clave

alpha 2 macroglobulin; alpha-macroglobulins; conformational change; electron microscopy; macroglobulins; protease inhibitor; protein conformation; protein cross-linking; small-angle X-ray scattering; structural model

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Péptido Hidrolasas / Alfa-Macroglobulinas Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Mol Cell Proteomics Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2021 Tipo del documento: Article País de afiliación: Dinamarca

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