Single-cell nuclear architecture across cell types in the mouse brain.
Science
; 374(6567): 586-594, 2021 Oct 29.
Article
en En
| MEDLINE
| ID: mdl-34591592
ABSTRACT
Diverse cell types in tissues have distinct gene expression programs, chromatin states, and nuclear architectures. To correlate such multimodal information across thousands of single cells in mouse brain tissue sections, we use integrated spatial genomics, imaging thousands of genomic loci along with RNAs and epigenetic markers simultaneously in individual cells. We reveal that cell typespecific association and scaffolding of DNA loci around nuclear bodies organize the nuclear architecture and correlate with differential expression levels in different cell types. At the submegabase level, active and inactive X chromosomes access similar domain structures in single cells despite distinct epigenetic and expression states. This work represents a major step forward in linking single-cell three-dimensional nuclear architecture, gene expression, and epigenetic modifications in a native tissue context.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
Corteza Cerebral
/
Neuroglía
/
Análisis de la Célula Individual
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos