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Membrane rigidity regulates E. coli proliferation rates.
Salinas-Almaguer, Samuel; Mell, Michael; Almendro-Vedia, Victor G; Calero, Macarena; Robledo-Sánchez, Kevin Carlo Martín; Ruiz-Suarez, Carlos; Alarcón, Tomás; Barrio, Rafael A; Hernández-Machado, Aurora; Monroy, Francisco.
Afiliación
  • Salinas-Almaguer S; Centro de Investigación y de Estudios Avanzados, Unidad Monterrey, Vía del Conocimiento 201, PIIT, 66600, Apodaca, NL, Mexico.
  • Mell M; Departamento de Química Física, Universidad Complutense de Madrid, Av. Complutense S/N, 28040, Madrid, Spain.
  • Almendro-Vedia VG; Departamento de Química Física, Universidad Complutense de Madrid, Av. Complutense S/N, 28040, Madrid, Spain.
  • Calero M; Departamento de Química Física, Universidad Complutense de Madrid, Av. Complutense S/N, 28040, Madrid, Spain.
  • Robledo-Sánchez KCM; Departamento de Química Física, Universidad Complutense de Madrid, Av. Complutense S/N, 28040, Madrid, Spain.
  • Ruiz-Suarez C; Translational Biophysics, Instituto de Investigación Sanitaria Hospital Doce de Octubre (IMAS12), Av. Andalucía S/N, 28041, Madrid, Spain.
  • Alarcón T; Centro de Investigación y de Estudios Avanzados, Unidad Monterrey, Vía del Conocimiento 201, PIIT, 66600, Apodaca, NL, Mexico.
  • Barrio RA; Centro de Investigación y de Estudios Avanzados, Unidad Monterrey, Vía del Conocimiento 201, PIIT, 66600, Apodaca, NL, Mexico.
  • Hernández-Machado A; ICREA, Pg. Lluís Companys 23, 08010, Barcelona, Spain.
  • Monroy F; Centre de Recerca Matemàtica, Edifici C, Campus de Bellaterra, 08193, Bellaterra, Barcelona, Spain.
Sci Rep ; 12(1): 933, 2022 01 18.
Article en En | MEDLINE | ID: mdl-35042922
Combining single cell experiments, population dynamics and theoretical methods of membrane mechanics, we put forward that the rate of cell proliferation in E. coli colonies can be regulated by modifiers of the mechanical properties of the bacterial membrane. Bacterial proliferation was modelled as mediated by cell division through a membrane constriction divisome based on FtsZ, a mechanically competent protein at elastic interaction against membrane rigidity. Using membrane fluctuation spectroscopy in the single cells, we revealed either membrane stiffening when considering hydrophobic long chain fatty substances, or membrane softening if short-chained hydrophilic molecules are used. Membrane stiffeners caused hindered growth under normal division in the microbial cultures, as expected for membrane rigidification. Membrane softeners, however, altered regular cell division causing persistent microbes that abnormally grow as long filamentous cells proliferating apparently faster. We invoke the concept of effective growth rate under the assumption of a heterogeneous population structure composed by distinguishable individuals with different FtsZ-content leading the possible forms of cell proliferation, from regular division in two normal daughters to continuous growing filamentation and budding. The results settle altogether into a master plot that captures a universal scaling between membrane rigidity and the divisional instability mediated by FtsZ at the onset of membrane constriction.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Membrana Celular / Proliferación Celular / Escherichia coli Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Membrana Celular / Proliferación Celular / Escherichia coli Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: México