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High Incidence of Lymph-node Metastasis in a Pancreatic-cancer Patient-derived Orthotopic Xenograft (PDOX) NOG-Mouse Model.
Sugisawa, Norihiko; Miyake, Kentaro; Higuchi, Takashi; Oshiro, Hiromichi; Park, Jun Ho; Kawaguchi, Kei; Bouvet, Michael; Unno, Michiaki; Hoffman, Robert M.
Afiliación
  • Sugisawa N; AntiCancer, Inc., San Diego, CA, U.S.A.
  • Miyake K; Department of Surgery, University of California, San Diego, CA, U.S.A.
  • Higuchi T; Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Oshiro H; AntiCancer, Inc., San Diego, CA, U.S.A.
  • Park JH; Department of Surgery, University of California, San Diego, CA, U.S.A.
  • Kawaguchi K; AntiCancer, Inc., San Diego, CA, U.S.A.
  • Bouvet M; Department of Surgery, University of California, San Diego, CA, U.S.A.
  • Unno M; AntiCancer, Inc., San Diego, CA, U.S.A.
  • Hoffman RM; Department of Surgery, University of California, San Diego, CA, U.S.A.
Anticancer Res ; 42(2): 739-743, 2022 Feb.
Article en En | MEDLINE | ID: mdl-35093872
BACKGROUND/AIM: Our laboratory pioneered the patient-derived orthotopic xenograft (PDOX) model. An important goal of PDOX-model development is facile visualization of metastasis in live mice. In the present report we evaluated tumor growth and metastasis in pancreatic cancer PDOX NOG [Non-obese diabetes (NOD)/Scid/IL2Rγnull]-and nude-mouse models using red fluorescent protein (RFP)-expressing tumor stroma to visualize the primary tumor and metastasis. MATERIALS AND METHODS: A patient-derived pancreatic cancer was initially implanted in transgenic RFP-expressing nude mice. Then, tumor fragments, which acquired RFP expressing stroma while growing in RFP-expressing nude mice were orthotopically implanted in nude and NOG mice. The primary pancreatic tumor and metastasis were observed 8 weeks after implantation. RESULTS: Lymph-node metastases expressing red fluorescence were detected only in NOG mice. Significantly faster growth of primary pancreatic tumors and a higher incidence of lymph-node metastasis occurred in NOG mice compared to nude mice. CONCLUSION: RFP-expressing tumor stroma, which traffics together with cancer cells to lymph nodes, is useful to observe tumor behavior, such as lymph-node metastasis in a PDOX NOG-mouse model which can be used for evaluation of novel anti-metastatic agents, as well as personalized therapy to identify effective drugs.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Modelos Animales de Enfermedad Tipo de estudio: Incidence_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Anticancer Res Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Modelos Animales de Enfermedad Tipo de estudio: Incidence_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Anticancer Res Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos