Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae.
Biotechnol Lett
; 44(4): 571-580, 2022 Apr.
Article
en En
| MEDLINE
| ID: mdl-35254611
ABSTRACT
Patchoulol is a natural sesquiterpene, which is widely used in perfumes and cosmetics. In the work, the mitochondria of S. cerevisiae were engineered for patchoulol production. The patchoulol titer of mitochondria-compartmentalized strain (1.79 mg/L) was 2.71-fold higher than that of control strain (0.66 mg/L) using genome-integrated patchoulol synthase, indicating that mitochondria compartmentation resulted in higher concentration of FPP (farnesyl pyrophosphate) precursor for patchoulol production. Moreover, when fused FPP synthase and patchoulol synthase was overexpressed in the strain with a mitochondria-localized DMAPP (dimethylallyl diphosphate) pathway, the production of patchoulol increased significantly to 19.24 mg/L, indicating more precursors were provided for patchoulol production. Nevertheless, the introduction of excess foreign proteins into mitochondria might cause a certain stress on mitochondria and showed a negative effect on the growth of yeast cells, which could hinder the expression of foreign pathways and reduce the patchoulol production. In conclusion, mitochondria-engineered yeast cells showed important potential for the enhanced biosynthesis of patchoulol, and further engineering could be considered based on the present work.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Sesquiterpenos
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
Biotechnol Lett
Año:
2022
Tipo del documento:
Article
País de afiliación:
China