Alginate and tunicate nanocellulose composite microbeads - Preparation, characterization and cell encapsulation.
Carbohydr Polym
; 286: 119284, 2022 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-35337506
ABSTRACT
Alginate has been used for decades for cell encapsulation. Cellulose nanofibrils (CNF) from tunicates are desirable in biomedicine due to high molecular weight, purity, crystallinity, and sustainable production. We prepared microbeads of 400-600 µm of alginate and tunicate CNF. Greater size, dispersity and aspect ratio were observed in microbeads with higher fractions of CNF. CNF content in Ca-crosslinked alginate microbeads decreased stability upon saline exposure, whereas crosslinking with calcium (50 mM) and barium (1 mM) yielded stable microbeads. The Young's moduli of gel cylinders decreased when exchanging alginate with CNF, and slightly increased permeability to dextran was observed in microbeads containing CNF. Encapsulation of MC3T3 cells revealed high cell viability after encapsulation (83.6 ± 0.4%) in beads of alginate and CNF. NHDFs showed lower viability but optimizing mixing and production techniques of microbeads increased cell viability (from 66.2 ± 5.3% to 72.7 ± 7.5%).
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Urocordados
/
Alginatos
Límite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Año:
2022
Tipo del documento:
Article
País de afiliación:
Noruega