Imaging Intracellular Trafficking in Neurons of C. elegans.

ABSTRACT

Axonal transport is an essential component of neuronal function. Several neurodegenerative disorders have been associated with defects in cargo transport. Thus, studying axonal transport is important to understand such disorders. Live imaging of fluorescently labeled cargo is a prevailing technique to study properties of axonal transport. C. elegans is both transparent and genetically amenable, making it an excellent model system to study axonal transport. In this chapter, we describe protocols to live image several neuronal cargo in vivo in C. elegans neurons.