An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines.

Kar, Bibekananda; Sabharwal, Ankit; Restrepo-Castillo, Santiago; Simone, Brandon W; Clark, Karl J; Ekker, Stephen C

Kar, Bibekananda; Sabharwal, Ankit; Restrepo-Castillo, Santiago; Simone, Brandon W; Clark, Karl J; Ekker, Stephen C.

Afiliación

Kar B; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Sabharwal A; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Restrepo-Castillo S; Mayo Clinic Graduate School of Biomedical Sciences, Virology and Gene Therapy Track, Mayo Clinic, Rochester, MN, USA.
Simone BW; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Clark KJ; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Ekker SC; Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.

STAR Protoc ; 3(2): 101288, 2022 06 17.

Article en En | MEDLINE | ID: mdl-35496789

Asunto(s)

ADN Mitocondrial; Mitocondrias; Línea Celular; ADN Mitocondrial/genética; Humanos; Mitocondrias/genética

Palabras clave

CRISPR; Cell Biology; Genetics; Molecular Biology; Sequencing

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Texto completo: 1 Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Mitocondrias Límite: Humans Idioma: En Revista: STAR Protoc Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

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