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Performance of islets of Langerhans conformally coated via an emulsion cross-linking method in diabetic rodents and nonhuman primates.
Stock, Aaron A; Gonzalez, Grisell C; Pete, Sophia I; De Toni, Teresa; Berman, Dora M; Rabassa, Alexander; Diaz, Waldo; Geary, James C; Willman, Melissa; Jackson, Joy M; DeHaseth, Noa H; Ziebarth, Noel M; Hogan, Anthony R; Ricordi, Camillo; Kenyon, Norma S; Tomei, Alice A.
Afiliación
  • Stock AA; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Gonzalez GC; Department of Biomedical Engineering, University of Miami, Miami, FL 33146, USA.
  • Pete SI; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • De Toni T; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Berman DM; Department of Biomedical Engineering, University of Miami, Miami, FL 33146, USA.
  • Rabassa A; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Diaz W; Department of Biomedical Engineering, University of Miami, Miami, FL 33146, USA.
  • Geary JC; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Willman M; Department of Surgery, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Jackson JM; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • DeHaseth NH; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Ziebarth NM; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Hogan AR; Diabetes Research Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Ricordi C; Department of Biomedical Engineering, University of Miami, Miami, FL 33146, USA.
  • Kenyon NS; Department of Biomedical Engineering, University of Miami, Miami, FL 33146, USA.
  • Tomei AA; Department of Biomedical Engineering, University of Miami, Miami, FL 33146, USA.
Sci Adv ; 8(26): eabm3145, 2022 07.
Article en En | MEDLINE | ID: mdl-35767620
Polyethylene glycol (PEG)-based conformal coating (CC) encapsulation of transplanted islets is a promising ß cell replacement therapy for the treatment of type 1 diabetes without chronic immunosuppression because it minimizes capsule thickness, graft volume, and insulin secretion delay. However, we show here that our original CC method, the direct method, requiring exposure of islets to low pH levels and inclusion of viscosity enhancers during coating, severely affected the viability, scalability, and biocompatibility of CC islets in nonhuman primate preclinical models of type 1 diabetes. We therefore developed and validated in vitro and in vivo, in several small- and large-animal models of type 1 diabetes, an augmented CC method-emulsion method-that achieves hydrogel CCs around islets at physiological pH for improved cytocompatibility, with PEG hydrogels for increased biocompatibility and with fivefold increase in encapsulation throughput for enhanced scalability.
Asunto(s)

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Diabetes Mellitus Tipo 1 Límite: Animals Idioma: En Revista: Sci Adv Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Trasplante de Islotes Pancreáticos / Islotes Pancreáticos / Diabetes Mellitus Tipo 1 Límite: Animals Idioma: En Revista: Sci Adv Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos