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Whole-genome sequencing and comparative analysis of Helicobacter pylori GZ7 strain isolated from China.
Zeng, Xiaoyan; Xiong, Lin; Wang, Wenling; Zhao, Yan; Xie, Yuan; Wang, Qinrong; Zhang, Qifang; Li, Leilei; Jia, Cencen; Liao, Yonghui; Zhou, Jianjiang.
Afiliación
  • Zeng X; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Xiong L; The Third Affiliated Hospital of Zunyi Medical University, The First People's Hospital of Zunyi), Fenghuang Road 98, Zunyi, 563099, China.
  • Wang W; GuiZhou Cancer Hospital, Beijing Road 9, Guiyang, 550004, China.
  • Zhao Y; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Xie Y; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Wang Q; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Zhang Q; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Li L; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Jia C; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Liao Y; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China.
  • Zhou J; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education and Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Beijing Road 9, GuizhouGuiyang, 550004, China. zjj1985048@gmc.edu.cn.
Folia Microbiol (Praha) ; 67(6): 923-934, 2022 Dec.
Article en En | MEDLINE | ID: mdl-35829852
ABSTRACT
Helicobacter pylori (H. pylori) is a Gram-negative pathogen as a carcinogen of the class Ι, with unique genetic diversity and wide geographic differences. The high incidence of gastric cancer in East Asia may be related to the bacterial genotype. It is of great significance that the genome of H. pylori in East Asia is widely collected. Therefore, we combined two sequencing technologies (PacBio and Illumina HiSeq 4000) and multiple databases to sequence and annotate the whole genome of H. pylori GZ7 isolated from a gastric cancer patient in Guizhou, China. Furthermore, this sequence was further compared with the genome sequence of 23 H. pylori strains isolated from different regions through collinearity comparison, specific gene analysis, phylogenetic tree construction, etc. The results showed that the genome of H. pylori GZ7 consists of 1,579,995 bp circle chromosomes with a GC content of 39.51%. This chromosome has 1,572 coding sequences, three antibiotic resistance genes, five prophages, and 198 virulence genes. The comparative genome analyses showed that H. pylori GZ7 has 53 specific genes compared to the other 23 strains. Most of these specific genes have not been annotated and characterized until now, whose research may provide insights into the biological activities of this strain. H. pylori GZ7 has the closest genetic relationship with H. pylori F30, and the farthest genetic relationship with H. pylori ELS37, which indicates that H. pylori genomes have geographical differences. This information may provide a molecular basis and guidance for constructing diagnostic methods for H. pylori and researching subsequent experiments.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Helicobacter pylori / Infecciones por Helicobacter Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Folia Microbiol (Praha) Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Helicobacter pylori / Infecciones por Helicobacter Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Folia Microbiol (Praha) Año: 2022 Tipo del documento: Article País de afiliación: China