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SCD2-mediated cooperative activation of IRF3-IRF9 regulatory circuit controls type I interferon transcriptome in CD4+ T cells.
Kanno, Toshio; Miyako, Keisuke; Nakajima, Takahiro; Yokoyama, Satoru; Sasamoto, Shigemi; Asou, Hikari K; Ohara, Osamu; Nakayama, Toshinori; Endo, Yusuke.
Afiliación
  • Kanno T; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Chiba, Japan.
  • Miyako K; Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan.
  • Nakajima T; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Chiba, Japan.
  • Yokoyama S; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Chiba, Japan.
  • Sasamoto S; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Chiba, Japan.
  • Asou HK; Department of Frontier Research and Development, Laboratory of Medical Omics Research, Kazusa DNA Research Institute, Chiba, Japan.
  • Ohara O; Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan.
  • Nakayama T; Department of Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan.
  • Endo Y; Japan Agency for Medical Research and Development (AMED) - Core Research for Evolutional Science and Technology (CREST), AMED, Chiba, Japan.
Front Immunol ; 13: 904875, 2022.
Article en En | MEDLINE | ID: mdl-36059459
Type I interferons (type I-IFN) are critical for the host defense to viral infection, and at the same time, the dysregulation of type I-IFN responses leads to autoinflammation or autoimmunity. Recently, we reported that the decrease in monounsaturated fatty acid caused by the genetic deletion of Scd2 is essential for the activation of type I-IFN signaling in CD4+ Th1 cells. Although interferon regulatory factor (IRF) is a family of homologous proteins that control the transcription of type I-IFN and interferon stimulated genes (ISGs), the member of the IRF family that is responsible for the type I-IFN responses induced by targeting of SCD2 remains unclear. Here, we report that the deletion of Scd2 triggered IRF3 activation for type I-IFN production, resulting in the nuclear translocation of IRF9 to induce ISG transcriptome in Th1 cells. These data led us to hypothesize that IRF9 plays an essential role in the transcriptional regulation of ISGs in Scd2-deleted (sgScd2) Th1 cells. By employing ChIP-seq analyses, we found a substantial percentage of the IRF9 target genes were shared by sgScd2 and IFNß-treated Th1 cells. Importantly, our detailed analyses identify a unique feature of IRF9 binding in sgScd2 Th1 cells that were not observed in IFNß-treated Th1 cells. In addition, our combined analyses of transcriptome and IRF9 ChIP-seq revealed that the autoimmunity related genes, which increase in patient with SLE, were selectively increased in sgScd2 Th1 cells. Thus, our findings provide novel mechanistic insights into the process of fatty acid metabolism that is essential for the type I-IFN response and the activation of the IRF family in CD4+ T cells.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Estearoil-CoA Desaturasa / Linfocitos T CD4-Positivos / Interferón Tipo I / Factores Reguladores del Interferón Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Front Immunol Año: 2022 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Estearoil-CoA Desaturasa / Linfocitos T CD4-Positivos / Interferón Tipo I / Factores Reguladores del Interferón Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Front Immunol Año: 2022 Tipo del documento: Article País de afiliación: Japón