ß2-microglobulin is overexpressed in buccal cells of elderly and correlated with expression of p16 and inflammatory genes.

ß2M (Beta 2 microglobulin) is a small protein that is found in all nucleated cells, previous finding showed that its levels increased in the serum of the elderly. Buccal cell samples are none invasive approach for assessing the expression of target genes. There was rationality to assess the expression of ß2M in buccal cells of people of a different group of ages. Indeed, the expression of ß2M increased significantly with fold change 3.40, 4.80, 6.60**, 8.20*** and 12.04*** for the group of age 18-25 years, 26-35 years, 36-45 years, 46-55 years, and 56-70 years respectively. The same observation was seen with markers of biological aging (p16INK4a) with fold change 3.19, 3.90, 4.80*, 8.50*** and 12.40*** for the group of age 18-25 years, 26-35 years, 36-45 years, 46-55 years, and 56-70 years respectively. As expected, there was an increase in the inflammatory genes (IL-1 ß and IL-6) expression in the elderly. Moreover, there was a direct significant correlation (r = 90, p < 0.001) between ß2M expression and age (years), and the same direct significant correlation between p16INK4a expression and age (years) was also seen (r = 90, p < 0.001). In addition, a direct correlation between ß2M and p16INK4a was also seen (r = 0.8.3, p < 0.001), there was also direct correlation between ß2M and IL-1 ß and IL-6 with (r = 0.5, p < 0.001; r = 0.68, p < 0.001) respectively. This evidence showed that ß2M increased in buccal cells of the elderly compared to younger, and thereby buccal cells can be exploited to assess biological aging by measuring ß2M levels, however, large sample size and using another assessing method such as ß2M protein levels should be performed to confirm the results.