Organ-on-a-chip with integrated semitransparent organic electrodes for barrier function monitoring.
Lab Chip
; 23(7): 1825-1834, 2023 03 28.
Article
en En
| MEDLINE
| ID: mdl-36810654
ABSTRACT
Organs-on-a-chip (OoC) are cell culture platforms that replicate key functional units of tissues in vitro. Barrier integrity and permeability evaluation are of utmost importance when studying barrier-forming tissues. Impedance spectroscopy is a powerful tool and is widely used to monitor barrier permeability and integrity in real-time. However, data comparison across devices is misleading due to the generation of a non-homogenous field across the tissue barrier, making impedance data normalization very challenging. In this work, we address this issue by integrating PEDOTPSS electrodes for barrier function monitoring with impedance spectroscopy. The semitransparent PEDOTPSS electrodes cover the entire cell culture membrane providing a homogenous electric field across the entire membrane making the cell culture area equally accountable to the measured impedance. To the best of our knowledge, PEDOTPSS has never been used solely to monitor the impedance of cellular barriers while enabling optical inspection in the OoC. The performance of the device is demonstrated by lining the device with intestinal cells where we monitored barrier formation under flow conditions, as well as barrier disruption and recovery under exposure to a permeability enhancer. The barrier tightness and integrity, and the intercellular cleft have been evaluated by analyzing the full impedance spectrum. Furthermore, the device is autoclavable paving the way toward more sustainable OoC options.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
/
Sistemas Microfisiológicos
Idioma:
En
Revista:
Lab Chip
Asunto de la revista:
BIOTECNOLOGIA
/
QUIMICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
España