Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling.
STAR Protoc
; 4(1): 102054, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36853719
ABSTRACT
Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared to rodent tissue due to greater extra-cellular matrix content and larger lipid droplets. In this protocol, we detail how to isolate nuclei from frozen subcutaneous human WAT for single-nuclei RNA-Seq. For complete information on the generation and use of this protocol, please refer to Whytock et al. (2022).1.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Grasa Subcutánea
/
Tejido Adiposo Blanco
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article