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An Adenosylcobalamin Specific Whole-Cell Biosensor.
Quispe Haro, Juan José; Wegner, Seraphine V.
Afiliación
  • Quispe Haro JJ; Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Waldeyerstrasse 15, 48149, Münster, Germany.
  • Wegner SV; Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Waldeyerstrasse 15, 48149, Münster, Germany.
Adv Healthc Mater ; 12(25): e2300835, 2023 10.
Article en En | MEDLINE | ID: mdl-37070155
Vitamin B12 (cobalamin) is essential for human health and its deficiency results in anemia and neurological damage. Vitamin B12 exists in different forms with various bioactivity but most sensors are unable to discriminate between them. Here, a whole-cell agglutination assay that is specific for adenosylcobalamin (AboB12), which is one of two bioactive forms, is reported. This biosensor consists of Escherichia coli that express the AdoB12 specific binding domain of CarH at their surface. In the presence of AdoB12, CarH forms tetramers, which leads to specific bacterial cell-cell adhesions and agglutination. These CarH tetramers disassemble upon green light illumination such that reversion of the bacterial aggregation can serve as internal quality control. The agglutination assay has a detection limit of 500 nм AdoB12, works in protein-poor biofluids such as urine, and has high specificity to AdoB12 over other forms of vitamin B12 as also demonstrated with commercially available supplements. This work is a proof of concept for a cheap and easy-to-readout AdoB12 sensor that can be implemented at the point-of-care to monitor high-dose vitamin B12 supplementation.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Técnicas Biosensibles Límite: Humans Idioma: En Revista: Adv Healthc Mater Año: 2023 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Técnicas Biosensibles Límite: Humans Idioma: En Revista: Adv Healthc Mater Año: 2023 Tipo del documento: Article País de afiliación: Alemania