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Identification of key microRNAs regulating ELOVL6 and glioblastoma tumorigenesis.
Istiqamah, Nurani; Matsuzaka, Takashi; Shimizu, Momo; Motomura, Kaori; Ohno, Hiroshi; Hasebe, Shiho; Sharma, Rahul; Okajima, Yuka; Matsuda, Erika; Han, Song-Iee; Mizunoe, Yuhei; Osaki, Yoshinori; Aita, Yuichi; Suzuki, Hiroaki; Sone, Hirohito; Takeuchi, Yoshinori; Sekiya, Motohiro; Yahagi, Naoya; Nakagawa, Yoshimi; Shimano, Hitoshi.
Afiliación
  • Istiqamah N; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Matsuzaka T; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Shimizu M; Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Motomura K; AMED-CREST, Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo, Japan.
  • Ohno H; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Hasebe S; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Sharma R; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Okajima Y; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Matsuda E; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Han SI; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Mizunoe Y; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Osaki Y; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Aita Y; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Suzuki H; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Sone H; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Takeuchi Y; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Sekiya M; Department of Internal Medicine, Faculty of Medicine, Niigata University, Niigata, Japan.
  • Yahagi N; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Nakagawa Y; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
  • Shimano H; Department of Endocrinology and Metabolism, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
BBA Adv ; 3: 100078, 2023.
Article en En | MEDLINE | ID: mdl-37082255
ABSTRACT
ELOVL fatty acid elongase 6 (ELOVL6) controls cellular fatty acid (FA) composition by catalyzing the elongation of palmitate (C160) to stearate (C180) and palmitoleate (C161n-7) to vaccinate (C181n-7). Although the transcriptional regulation of ELOVL6 has been well studied, the post-transcriptional regulation of ELOVL6 is not fully understood. Therefore, this study aims to evaluate the role of microRNAs (miRNAs) in regulating human ELOVL6. Bioinformatic analysis identified five putative miRNAs miR-135b-5p, miR-135a-5p, miR-125a-5p, miR-125b-5p, and miR-22-3p, which potentially bind ELOVL6 3'-untranslated region (UTR). Results from dual-luciferase assays revealed that these miRNAs downregulate ELOVL6 by directly interacting with the 3'-UTR of ELOVL6 mRNA. Moreover, miR-135b-5p and miR-135a-5p suppress cell proliferation and migration in glioblastoma multiforme cells by inhibiting ELOVL6 at the mRNA and protein levels. Taken together, our results provide novel regulatory mechanisms for ELOVL6 at the post-transcriptional level and identify potential candidates for the treatment of patients with glioblastoma multiforme.
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Texto completo: 1 Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: BBA Adv Año: 2023 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: BBA Adv Año: 2023 Tipo del documento: Article País de afiliación: Japón