m6Amediated LINC02038 inhibits colorectal cancer progression via regulation of the FAM172A/PI3K/AKT pathway via competitive binding with miR5525p.
Int J Oncol
; 63(1)2023 07.
Article
en En
| MEDLINE
| ID: mdl-37264959
ABSTRACT
Long noncoding RNAs (lncRNAs) are a type of regulatory molecule with potential roles in the development of several different malignancies. However, the underlying mechanisms of lncRNAs in colorectal cancer (CRC) are incompletely understood. The present study investigated the molecular mechanism of LINC02038 in CRC. LINC02038 expression was decreased in CRC tissues compared to the paracancerous tissues and LINC02038 overexpression markedly reduced the proliferation, vitality, migration and invasive ability and greatly accelerated apoptosis of colorectal cancer cells. Bioinformatics examination indicated that LINC02038 may have targeted microRNA (miR)5525p. RNA immunoprecipitation and luciferase reporter assays showed that LINC02038 served as a sponge for miR5525p, hindering target gene FAM172A of miR5525p degradation. Moreover, methylated RNA immunoprecipitation (MeRIP)qualitative PCR assays revealed that YTHDF2 could identify and regulate the METTL3mediated LINC02038 N6methyladenosine (m6A) modification and increase its degradation, thereby promoting CRC progression via the PI3K/AKT pathway. Based on the CRC clinical specimens, it was shown that LINC02038 was negatively associated with lymphatic metastasis and distant metastasis. These results revealed that m6A/LINC02038/miR5525p/FAM172A may be a novel antitumor axis and LINC02038 may serve as a biomarker and treatment option for colorectal cancer.
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Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
MicroARNs
/
ARN Largo no Codificante
Tipo de estudio:
Qualitative_research
Límite:
Humans
Idioma:
En
Revista:
Int J Oncol
Asunto de la revista:
NEOPLASIAS
Año:
2023
Tipo del documento:
Article