Optimization of reversed-phase solid-phase extraction for shotgun proteomics analysis.
J Mass Spectrom
; 58(8): e4965, 2023 Aug.
Article
en En
| MEDLINE
| ID: mdl-37464559
ABSTRACT
Reversed-phase solid-phase extraction (SPE) is the method of choice for the purification of proteomics samples. Even though the efficacy of SPE methods is sample type-dependent, the manufacturers' protocols are used in most studies. Using an optimized SPE method can lead to a substantial gain in identification and recovery. In this tutorial, we give a brief introduction to the most important parameters influencing SPE performance, and we present a short workflow (16 measurements) for optimizing the SPE procedure. This is complemented by method performance assessment instructions and a short troubleshooting guide to help users further understand and investigate their SPE methods.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Proteómica
/
Extracción en Fase Sólida
Idioma:
En
Revista:
J Mass Spectrom
Asunto de la revista:
BIOQUIMICA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Hungria