Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity.
Nat Biotechnol
; 2023 Aug 03.
Article
en En
| MEDLINE
| ID: mdl-37537501
ABSTRACT
Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5-9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins.
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Base de datos:
MEDLINE
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Francia