Unveiling the structural mechanism of a G-quadruplex pH-Driven switch.
Biochimie
; 214(Pt A): 73-82, 2023 Nov.
Article
en En
| MEDLINE
| ID: mdl-37573019
ABSTRACT
The human telomere oligonucleotide, d[TAGGG(TTAGGG)2TTAGG] (TAGGG), can adopt two distinct 2-G-quartet G-quadruplex structures at pH 7.0 and 5.0, referred to as the TD and KDH+ forms, respectively. By using a combination of NMR and computational techniques, we determined high-resolution structures of both forms, which revealed unique loop architectures, base triples, and base pairs that play a crucial role in the pH-driven structural transformation of TAGGG. Our study demonstrated that TAGGG represents a reversible pH-driven switch system where the stability and pH-induced structural transformation of the G-quadruplexes are influenced by the terminal residues and base triples. Gaining insight into the factors that regulate the formation of G-quadruplexes and their pH-sensitive structural equilibrium holds great potential for the rational design of novel DNA based pH-driven switches. These advancements in understanding create exciting opportunities for applications in the field of nanotechnology, specifically in the development of bio-nano-motors.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
G-Cuádruplex
Límite:
Humans
Idioma:
En
Revista:
Biochimie
Año:
2023
Tipo del documento:
Article
País de afiliación:
Eslovenia