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Low-glucose culture environment can enhance the wound healing capability of diabetic adipose-derived stem cells.
Li, Chun-Wei; Young, Tai-Horng; Wang, Mu-Hui; Pei, Ming-Ying; Hsieh, Tsung-Yu; Hsu, Chia-Lang; Cheng, Nai-Chen.
Afiliación
  • Li CW; Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital at Keelung, Chang Gung University and College of Medicine, Keelung, Taiwan.
  • Young TH; Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
  • Wang MH; Department of Surgery, National Taiwan University Hospital and College of Medicine, 7 Chung-Shan S. Rd., Taipei, 100, Taiwan.
  • Pei MY; Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
  • Hsieh TY; Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan.
  • Hsu CL; Department of Medical Research, National Taiwan University Hospital, Taipei, Taiwan.
  • Cheng NC; Department of Surgery, National Taiwan University Hospital and College of Medicine, 7 Chung-Shan S. Rd., Taipei, 100, Taiwan. nccheng@ntu.edu.tw.
Stem Cell Res Ther ; 14(1): 236, 2023 09 04.
Article en En | MEDLINE | ID: mdl-37667384
ABSTRACT

BACKGROUND:

Application of autologous adipose-derived stem cells (ASC) for diabetic chronic wounds has become an emerging treatment option. However, ASCs from diabetic individuals showed impaired cell function and suboptimal wound healing effects. We proposed that adopting a low-glucose level in the culture medium for diabetic ASCs may restore their pro-healing capabilities.

METHODS:

ASCs from diabetic humans and mice were retrieved and cultured in high-glucose (HG, 4.5 g/L) or low-glucose (LG, 1.0 g/L) conditions. Cell characteristics and functions were investigated in vitro. Moreover, we applied diabetic murine ASCs cultured in HG or LG condition to a wound healing model in diabetic mice to compare their healing capabilities in vivo.

RESULTS:

Human ASCs exhibited decreased cell proliferation and migration with enhanced senescence when cultured in HG condition in vitro. Similar findings were noted in ASCs derived from diabetic mice. The inferior cellular functions could be partially recovered when they were cultured in LG condition. In the animal study, wounds healed faster when treated with HG- or LG-cultured diabetic ASCs relative to the control group. Moreover, higher collagen density, more angiogenesis and cellular retention of applied ASCs were found in wound tissues treated with diabetic ASCs cultured in LG condition.

CONCLUSIONS:

In line with the literature, our study showed that a diabetic milieu exerts an adverse effect on ASCs. Adopting LG culture condition is a simple and effective approach to enhance the wound healing capabilities of diabetic ASCs, which is valuable for the clinical application of autologous ASCs from diabetic patients.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Diabetes Mellitus Experimental Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2023 Tipo del documento: Article País de afiliación: Taiwán

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Diabetes Mellitus Experimental Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2023 Tipo del documento: Article País de afiliación: Taiwán