Unraveling the role of intra-cellular metabolites in the lactic acid production by novel Bacillus amyloliquefaciens using sugarcane molasses as a substratum.
Mol Omics
; 20(1): 19-26, 2024 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-37691617
ABSTRACT
Lactic acid is a versatile, multi-functional organic monomer in various industries, creating worldwide demand. High titer lactic acid production was achieved by novel Bacillus amyloliquefaciens J2V2AA through sugarcane molasses fermentation up to 178 mg mL-1. A metabolomics approach such as combined GC-MS and LC-MS was applied to elucidate the involvement of key metabolites in lactic acid production. The results revealed the participation of 58 known intra-cellular metabolites at various pathways in lactic acid production. Twenty-eight highly up-regulated and down-regulated metabolites were analyzed, and a schematic diagram of a possible lactic acid production pathway was proposed. The produced lactic acid was analyzed through FTIR, UV-Spectrum, and HPLC analysis.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Saccharum
/
Bacillus amyloliquefaciens
Idioma:
En
Revista:
Mol Omics
Año:
2024
Tipo del documento:
Article
País de afiliación:
India