Analysis of full and empty ratio of EV71 virus by using capillary zone electrophoresis.
Electrophoresis
; 45(3-4): 327-332, 2024 Feb.
Article
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| MEDLINE
| ID: mdl-38010589
ABSTRACT
Hand, foot, and mouth disease is a serious public health problem, and the main pathogen is enterovirus 71 (EV71). Its capsid assembly mechanism including capsid protein processing has been widely studied. Full and empty capsids have different immunological efficacy. Therefore, tracking full/empty capsid ratio throughout the EV71 production process is important to ensure consistent product quality and proper dosing response. The analysis of full/empty capsid ratio of intact virus has been widely reported as well. A variety of techniques have been employed to evaluate the full/empty capsid ratios. However, there has not been a rapid, reproducible, and robust assay to determine the full/empty capsid ratios of final and in-process products. In this study, a novel assay based on capillary zone electrophoresis was established. The separation of full and empty species could be achieved within 10 min and the ratio of peak areas was used to calculate the full/empty capsid ratio directly. The results showed good reproducibility and linearity for the determination of full/empty capsid ratios.
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Base de datos:
MEDLINE
Asunto principal:
Enterovirus Humano A
Idioma:
En
Revista:
Electrophoresis
Año:
2024
Tipo del documento:
Article