Diversity of biofilm-specific antimicrobial resistance genes in Pseudomonas aeruginosa recovered from various clinical isolates.
Iran J Microbiol
; 15(6): 742-749, 2023 Dec.
Article
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| MEDLINE
| ID: mdl-38156300
ABSTRACT
Background and Objectives:
The resistance of Pseudomonas aeruginosa to antibiotics offers a significant challenge in the treatment of patients. This study aimed to investigate the antimicrobial resistance profile, biofilm-specific antimicrobial resistance genes, and genetic diversity of P. aeruginosa recovered from clinical samples. Materials andMethods:
Totally 47 non-duplicate isolates of P. aeruginosa were recovered from various clinical samples. toxA, algD, ndvB, and tssC1 genes were detected in biofilm-producing isolates. The DNA sequences of the toxA and tssC1 genes were analyzed, by creating phylogenetic trees.Results:
The findings revealed that 30 (63.8%) of the isolates tested positive for Extended spectrum ß-lactamase (ESBL), whereas 31 (65.9%) tested positive for Metallo-ß-lactamase (MBL) and all of the isolates presented the toxA genes, and 19.1%,17%, 6.3% presented by algD, ndvB and tssC1 genes. Besides, the phylogenetic trees of the toxA and tssC1 gene isolates suggested a genotype that was closely aligned with others. Gene sequencing similarity revealed 99% identity with other isolates deposited in GenBank.Conclusion:
The occurrence of toxA was most prevalent. One isolate was recorded as a novel isolate in the global gene bank as a locally isolated strain from the city of Erbil that has never been identified in global isolates due to genetic variation.
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Base de datos:
MEDLINE
Idioma:
En
Revista:
Iran J Microbiol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Irak