Alignment, calibration, and validation of an adaptive optics scanning laser ophthalmoscope for high-resolution human foveal imaging.
Appl Opt
; 63(3): 730-742, 2024 Jan 20.
Article
en En
| MEDLINE
| ID: mdl-38294386
ABSTRACT
In prior art, advances in adaptive optics scanning laser ophthalmoscope (AOSLO) technology have enabled cones in the human fovea to be resolved in healthy eyes with normal vision and low to moderate refractive errors, providing new insight into human foveal anatomy, visual perception, and retinal degenerative diseases. These high-resolution ophthalmoscopes require careful alignment of each optical subsystem to ensure diffraction-limited imaging performance, which is necessary for resolving the smallest foveal cones. This paper presents a systematic and rigorous methodology for building, aligning, calibrating, and testing an AOSLO designed for imaging the cone mosaic of the central fovea in humans with cellular resolution. This methodology uses a two-stage alignment procedure and thorough system testing to achieve diffraction-limited performance. Results from retinal imaging of healthy human subjects under 30 years of age with refractive errors of less than 3.5 diopters using either 680 nm or 840 nm light show that the system can resolve cones at the very center of the fovea, the region where the cones are smallest and most densely packed.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de la Retina
/
Oftalmoscopios
/
Fóvea Central
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Appl Opt
Año:
2024
Tipo del documento:
Article