Harnessing HetHydrogel: A Universal Platform to Dropletize Single-Cell Multiomics.
Small Methods
; 8(7): e2301631, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38419597
ABSTRACT
A universal platform is developed for dropletizing single cell plate-based multiomic assays, consisting of three main pillars a miniaturized open Heterogeneous Hydrogel reactor (abbreviated HetHydrogel) for multi-step biochemistry, its tunable permeability that allows Tn5 tagmentation, and single cell droplet barcoding. Through optimizing the HetHydrogel manufacturing procedure, the chemical composition, and cell permeation conditions, simultaneous high-throughput mitochondrial DNA genotyping and chromatin profiling at the single-cell level are demonstrated using a mixed-species experiment. This platform offers a powerful way to investigate the genotype-phenotype relationships of various mtDNA mutations in biological processes. The HetHydrogel platform is believed to have the potential to democratize droplet technologies, upgrading a whole range of plate-based single cell assays to high throughput format.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
ADN Mitocondrial
/
Hidrogeles
/
Análisis de la Célula Individual
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Small Methods
Año:
2024
Tipo del documento:
Article
País de afiliación:
China