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Variability of BMP-2 content in DBM products derived from different long bone.
Zhao, Yong-Jie; Yin, Gang; Liu, Bin; Deng, Xiao-Qiang; Cao, Hai-Yan; Liu, Ying.
Afiliación
  • Zhao YJ; Binzhou Medical University Hospital, Binzhou, Shandong Province, China.
  • Yin G; Binzhou Medical University Hospital, Binzhou, Shandong Province, China.
  • Liu B; Binzhou Medical University Hospital, Binzhou, Shandong Province, China.
  • Deng XQ; Xing'an League People's Hospital, Ulanhot, Inner Mongolia, China.
  • Cao HY; Binzhou Medical University Hospital, Binzhou, Shandong Province, China.
  • Liu Y; Binzhou Medical University Hospital, Binzhou, Shandong Province, China. Lyingdoctor@163.com.
Cell Tissue Bank ; 25(2): 697-703, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38489016
ABSTRACT
Demineralized bone matrix (DBM) has been regarded as an ideal bone substitute as a native carrier of bone morphogenetic proteins (BMPs) and other growth factors. However, the osteoinductive properties diverse in different DBM products. We speculate that the harvest origin further contributing to variability of BMPs contents in DBM products besides the process technology. In the study, the cortical bone of femur, tibia, humerus, and ulna from a signal donor were prepared and followed demineralizd into DBM products. Proteins in bone martix were extracted using guanidine-HCl and collagenase, respectively, and BMP-2 content was detected by sandwich enzyme-linked immunosorbent assay (ELISA). Variability of BMP-2 content was found in 4 different DBM products. By guanidine-HCl extraction, the average concentration in DBMs harvested from ulna, humerus, tibia, and femur were 0.613 ± 0.053, 0.848 ± 0.051, 3.293 ± 0.268, and 21.763 ± 0.344, respectively (p < 0.05), while using collagenase, the levels were 0.089 ± 0.004, 0.097 ± 0.004, 0.330 ± 0.012, and 1.562 ± 0.008, respectively (p < 0.05). In general, the content of BMP-2 in long bones of Lower limb was higher than that in long bones of upper limb, and GuHCl had remarkably superior extracted efficiency for BMP-2 compared to collagenase. The results suggest that the origin of cortical bones harvested to fabricate DBM products contribute to the variability of native BMP-2 content, while the protein extracted method only changes the measured values of BMP-2.
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Texto completo: 1 Base de datos: MEDLINE Asunto principal: Matriz Ósea / Proteína Morfogenética Ósea 2 Límite: Humans Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Base de datos: MEDLINE Asunto principal: Matriz Ósea / Proteína Morfogenética Ósea 2 Límite: Humans Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: China