Isolation and identification of rhizospheric pseudomonads with insecticidal effects from various crops in Khuzestan Province, Iran.

ABSTRACT

Pseudomonas bacteria include a variety of species with distinct characteristics. Some species within this genus are known for their ability to stimulate plant growth. Recently, the potential of these bacteria in controlling insect pests has been documented. In this study, 58 bacterial isolates were purified from rhizospheres of wheat, broad bean and canola that were collected from different fields of Khuzestan province in south-west of Iran. With biochemical tests 19 non plant pathogenic pseudomonads strains were detected and their lethal effects on the eggs and larvae of Ephestia keuhniella as an important pest that infests stored products, were evaluated under laboratory conditions. For the bioassays, two concentrations of each strain were administered, and the 5th instar larvae and eggs of the pest were subjected to treatment. Mortality rates were recorded after 24 h. The results showed that all isolated Pseudomonad strains of this study had insecticidal effects against eggs and larvae of E. keuhniella. The strains AWI1, AWI2, AWI7, ABI12, ABI15 and ABI16 displayed the highest mortality rate (91.1 %, 86.2 %, 82.3 %, 84.2, 90.5 % and 90.5 %, respectively). Molecular identification and phylogeny tree according to 16 s rRNA sequencing clarified that AWI1, AWI2 belong to P. plecoglossicida, AWI5 belongs to P. lini, ABI12, ABI15 and ABI16 belong to P. taiwanensis. Moreover, the bacterial efficacy at a suspension concentration of 0.5 OD (80 %) was significantly greater than that at a concentration of 0.2 OD (63.33 %). No significant difference was detected in the response of the pest larvae or eggs to the different strains. Furthermore, olfactory trials revealed that the female parasitoid wasp Habrabracon hebetor actively avoided the infection of the treated larvae by the strains. These findings have practical implications for the development of microbiological pest control strategies.