Protocol for unsupervised inference of cell-cell communication using matrix decomposition.
STAR Protoc
; 5(2): 103006, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38602871
ABSTRACT
Exploring cell-cell communication is pivotal for understanding biological processes in multicellular life forms. Here, we present a protocol that details the use of matrix decomposition to infer cell-cell communication (MDIC3) for unsupervised cell-cell communication inference. We describe steps for using the MDIC3 Python scripts to deduce cell-cell communication and identify key ligand-receptor pairs between a specific cell type pair from a single-cell gene expression dataset. This protocol has potential application in cell-cell communication inference on any species. For complete details on the use and execution of this protocol, please refer to Liu et al.1.
Palabras clave
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Comunicación Celular
Límite:
Animals
/
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
China